![]() The peaks in the beginning are distinct and readable but they become overlapping at around 100 bp. Is the sequence below of good quality? No. Even though the computer is "calling" the bases, the peaks are overlapping and not distinct. ![]() Is the sequence below of good quality? Yes! The peaks are symmetic, evenly spaced, nonoverlapping and greater than 300 bases. There is over 300 bp of good quality sequence. The DNA waveform peaks are non-overlapping Unreadable Which clone status indicates that you have correctly completed the analysis of a Practice Clone (PC)? (Check all that apply) Reviewed and Correct If you are stuck on specific step in DSAP where is the first place you should look for help? The Help (lifesaver) icon in DSAP If you are stuck on specific step in DSAP, and the Help links within DSAP do not provide an answer, where is the next place you should look for help? The Clone Discussion link in DSAP How are the "quality scores" indicated in 4Peaks? Gray city landscapes How are the "quality scores" indicated in FinchTV and SnapGene? Bars What are the criteria for determining if your sequence is of good quality? (Check all that apply) The DNA waveform peaks for the most part are symmetric Make sure that you are logging into the correct DSAP Which type of clones do you need to work on to gain practice on analyzing a cDNA sequence from the cDNA library PC What is the best way to contact the WSSP staff if you have questions about a specific clone? Use the DSAP Clone Discussion What "clone status" means that you have to make corrections on the analysis of one of your clones? Reviewed, needs to be reanalyzed by student Which clone status indicates that you have correctly completed the analysis of an Unknown clone? (Check all that apply) To be submitted to NCBI To provide a mechanism to contact students and staff about questions in the analysis of specific DNA sequences When registering on DSAP for the first time or logging into the program after you have registered, what is very important? (check all that apply) You remember your username and password! To review students' answers and return them if they need corrections ![]() To provide help information for students to analyze their DNA sequences. To record students' answers in analysis of of their DNA sequences What would cause the small black peak under the second T peak? The DNA polymerase skipped a base during synthesis What is the first step in the analysis of a cDNA sequence from the Landolita library? Determine if the waveform is of good quality What is the function of DSAP? (check all that apply) To guide students through the analysis of their clones ![]()
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